DOT1L in Human Lung. DOT1L was detected in immersion fixed paraffin­ embedded sections of human lung using Human DOT1L Monoclonal Antibody at 15ug/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat­induced epitope retrieval using Antigen Retrieval Reagent­Basic. Tissue was stained using the Mouse HRP­DAB Cell & Tissue Staining Kit a n d counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin­embedded Tissue Sections.